Isolation and characterization of glyoxylate dehydrogenase from the fungus Sclerotium rolfsii.
نویسندگان
چکیده
Glyoxylate dehydrogenase (glyoxylate:NAD+ oxidoreductase) was purified 600-fold in three steps from crude extracts of the fungus Sclerotium rolfsii (Corticium rolfsii Curzi). Two of the purification steps involved dye-affinity chromatography. The enzyme is a tetramer of Mr 250 000, with identical subunits of Mr 57 000. Inhibition studies suggest that there is one essential thiol group per active site.
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 218 1 شماره
صفحات -
تاریخ انتشار 1984